Skrining Dan Evaluasi Aktivitas Kitinase Dan Produksi N-Asetilglukosamin Dari Sembilan Isolat Bakteri Lokal

Kitinase adalah suatu poli 1,4-β (2-asetamido-2-deoksi-D-glukosaminida), merupakan enzim yang mendegradasi kitin pada ikatan β-1,4-asetamido-2-deoksi-D-glikosida menjadi produk turunannya yaitu kitin oligosakaridaatau monomer NAG. Enzim kitinase dihasilkan oleh mikroorganisme kitinolitik yang sebagian besar terdapat di lingkungan tanah dan air. Penelitian kali ini bertujuan untuk skrining dan evaluasi kemampuan 9 bakteri isolat dalam menghasilkan kitinase. Isolat yang terbaik adalah isolat yang menghasilkan kitnase dengan aktivitas yang tinggi dan mampu menghidrolisis kitin menjadi monomer NAG. Dari 9 isolat bakteri tersebut bakteri BPPTCC-2 merupakan bakteri yang terpilih karena mampu menghasilkan kitinase dengan aktivitas tertinggi yaitu 0,1780 U/mL . kitinase tersebut dapat menghidrolisis substrat kitin dan menghasilkan NAG sebagai produk tunggal dan dengan rendemen NAG paling tinggi yaitu 11,5%

ONCOCYTOMA OF THE NASAL CAVITY

ABSTRACT Oncocytoma of the nasal cavity is an extremely rare condition with only a few cases reported in the literature. A case of oncocytoma of the nasal cavity in a 12 years old Nepali boy is presented along with a brief review of the relevant literature. Key Words: Oncocytoma, Oxyphil adenoma, Nasal cavity

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Not AvailableBiotic communities are sensitive to environmental factors and offer an integral perspective of the environmental conditions of a given habitat. Fish are widely used as sentinel species to assess environmental health and are excellent indicators of overall health of rivers. A study was carried out to evaluate and compare the environmental quality of different stretches of River Mahanadi by analyzing fish health using Health Assessment Index (HAI). The HAI values of individual fishes varied from 0 to 200. The fish which got the highest score for HAI was from the winter sample of Sonepur. The lowest score for HAI was recorded at Tikarpara, a site which falls under protected Satkosia river sanctuary. The most common groups of parasites recorded were myxozoans, trichodinids, copepods and monogenetic trematodes. Parasite numbers recorded from Cirrhinus reba supported the HAI hypothesis that higher endoparasite and lower ectoparasite numbers occur at highly impacted areas. The present study suggests that HAI can be used as an effective biological monitoring tool for Indian rivers.Not Availabl

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Not Availableroadmap for fisheries development in mauns and chaurs in BiharNot Availabl

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Not AvailableSerum immunoglobulins (Ig) of mrigal Cirrhinus mrigala (Hamilton 1822) immunised with bovine serum albumin (BSA), were purified by affinity chromatography using BSA-CL agarose column. The purified mrigal Ig (m-Ig) was characterised under reducing condition by Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) which revealed two bands of 85 and 26 kDa corresponding to heavy and light chain, respectively. Following fusion of splenocytes from Balb/c mice immunised with purified m-Ig with myeloma cells, three hybridomas showing reactivity with m-Ig were cloned by limiting dilution. The monoclonal antibodies (MAbs) generated by these clones were designated as 3B2-E12, 3B2-F9 and 4C3-B2 and characterised by western blotting and isotyping. Western blot analysis of the supernatant from the three clones with purified m-Ig indicated that, all the three MAbs were specific to heavy chain. Isotyping revealed that 3B2-E12 MAb was of IgG1 isotype whereas the other two MAbs were of IgG2a isotype. Cross reactivity of anti-mrigal Ig MAb (3B2-E12) was observed with serum Ig of Catla catla and Labeo rohita indicating semi-conserved nature of Ig in Indian major carps.Not Availabl

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Not AvailableStudies on Sisorinae systematics have been largely restricted to morphological data with few studies on examination of phylogenetic relations. However, no study has been done to evaluate genetic distance of the genera under Sisorinae sub-family and detailed phylogenetic relations within it. We used nuclear recombination activating 2 (rag2) gene and mitochondrial cytochrome c oxidase I (COI) gene from 64 species to examine genetic differentiation and phylogenetic relationships within 11 Asian Sisorinae genera. The range of interspecies K2P distance for rag2 was 0–0.061 and COI was 0–0.204. Phylogenetic analysis based on maximum likelihood (ML) and Bayesian (BI) approaches for each locus individually and for the concatenated rag2 and COI sequences revealed three major subclades viz. Bagariini, Sisorini and Erethistini under subfamily Sisorinae. The analysis based on COI gene showed ((Sisorini, Bagariini), Erethistini) relationship. Rag2 and combined rag2 and COI showed ((Sisorini, Erethistini), Bagariini) relationship. Combined rag2 and COI analyses resulted into better resolved trees with a good bootstrap support. In this study, new record of Pseudolaguvia foveolata (Erethistini) has been documented based on 13 specimens collected from Torsa River, Jaldapara, Alipurduar district, West Bengal, India (26°43′44.66″ N and 89°19′32.34″ E), extending its distribution range in Brahmaputra drainage, India. The genetic distance between the P. foveolata new record and the reported P. foveolata (holotype: UMMZ 244867) was 0.00 at both rag2 and COI locus and it was further grouped with P. foveolata Type specimen (holotype: UMMZ 244867) with 100% bootstrap support. This report gives additional information on occurrence of the species P. foveolata, along with discussion on morphometric, meristic and molecular (COI and rag2 gene) data.Not Availabl